Employees at Walt Disney Studios tour South America, drawing along the way as they soak up South American culture, and telling us four stories: Lake Titicaca, Pedro, El Gaucho Goofy, and Aquarela do Brasil.
- Donald Duck and José Carioca first met in Brazil.
- During the events of Aquarela do Brasil, Donald carried a card with a black spade on one side and his information on the other side. The card indicated he lived in Hollywood. José carried a similar card which gave his home as Rio de Jainero, Brasil.
- The canonicity of the Aquarela do Brasil is questionable, as it depicts Donald Duck and José Carioca as being painted into existence as opposed to being flesh-and-blood beings. Of course, it is worthy to note that this concept does fit with the concept of toons and Toon versions of both Donald and José appear in Who Framed Roger Rabbit. Thus, the argument could be made that Aquarela do Brasil depicts the births of Toon Donald and Toon José, not the flesh-and-blood versions of them who descended from others birds, not just a paintbrush. It's also possible that the events of the short did happen to the flesh-and-blood Donald and José, but that the sequences involving the painter are non-canonical and are a storytelling method rather than a concrete fact.
- The Three Caballeros (1944) is something of a sequel to this film. It is also a film that honors Latin American culture and uses Donald Duck as something of a symbol for the American viewer: eagerly learning and experiencing the wonders of Latin America. José Carioca also reappears in The Three Caballeros, and he already knows Donald, confirming that the film takes place chronologically after Saludos Amigos or, at least, the Aquarela do Brasil segment of Saludos Amigos, where Donald and José are shown meeting each other for the first time.
- Donald references the events of Aquarela do Brasil in Two Happy Amigos (1960), saying to José, "Hey, remember when you taught me the samba?"
- Legend of the Three Caballeros (2018) plays itself as something of a sequel to this film, seemingly taking place in between Saludos Amigos and The Three Caballeros. This can be deduced as Donald and José do not feel the introduce themselves to each other during the events of Dope-a-Cabana, yet Panchito Pistoles does introduce himself to both, indicating that the show depicts Panchito's first meeting of Donald and José but may or may not depict Donald and José's first meeting of each other. It would make sense that Donald and José met prior to Dope-a-Cabana as Saludos Amigos clearly shows them as meeting during the events of Aquarela do Brasil.
Behind the scenes
Saludos Amigos is based on the real-life tour Walt Disney and roughly twenty of his studio members took through South America. It consists of footage, narration, and some animation explaining the tour. In addition, four short animated stories or segments are used to portray various aspects of South American culture: Lake Titicaca, Pedro, Gaucho Goofy, and Aquarela do Brasil.